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一本蛋白质工程的书-Invitro Mutagenesis Protocols,此书也是methods in molecular biology的一卷(Volume182),主要讲的是突变方法,也就是突变文库的构建,不过呢是以具体实例讲的,比较详细,做蛋白质工程的好好看看哦。


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Contents
Dedication .........................................................................................................v
Preface ........................................................................................................... vii
Contributors ................................................................................................... xiii
1 Rapid and Reliable Site-Directed Mutagenesis
Using Kunkel's Approach
Priya Handa, Swapna Thanedar,
and Umesh Varshney ........................................................................ 1
2 Site-Directed Mutagenesis Using Altered
β-Lactamase Specificity
Christine A. Andrews and Scott A. Lesley ......................................... 7
3 Site-Directed Mutagenesis Facilitated by DpnI Selection
on Hemimethylated DNA
Fusheng Li and James I. Mullins ....................................................... 19
4 Multiple Site-Directed Mutagenesis In Vitro
Yang-Gyun Kim and Stefan Maas ...................................................... 29
5 Two-Stage Polymerase Chain Reaction Protocol Allowing
Introduction of Multiple Mutations, Deletions, and Insertions,
Using QuikChange™ Site-Directed Mutagenesis
Wenyan Wang and Bruce A. Malcolm ............................................... 37
6 Efficient and Accurate Site-Directed Mutagenesis
of Large Plasmids
Susan A. Nadin-Davis ......................................................................... 45
7 Combining Site-Specific Chemical Modification with Site-Directed
Mutagenesis: Versatile Strategy to Move Beyond Structural
Limitations of 20 Natural Amino Acids Side Chains
in Protein Engineering
Grace DeSantis and J. Bryan Jones ................................................. 55
8 Site-Directed Mutagenesis Mediated by a Single Polymerase
Chain Reaction Product
Xueni Chen, Weimin Liu, Ileana Quinto,
and Giuseppe Scala ........................................................................ 67
9 Megaprimer Method for Polymerase Chain Reaction-Mediated
Generation of Specific Mutations in DNA
Jesper Brøns-Poulson, Jane Nøhr,
and Leif Kongskov Larsen ............................................................. 71
10 Generation of Epitope-Tagged Proteins by Inverse
Polymerase Chain Reaction Mutagenesis
Lucio Gama and Gerda E. Breitwieser .............................................. 77
11 Site-Directed Mutagenesis by Polymerase Chain Reaction
Albert Jeltsch and Thomas Lanio ..................................................... 85
12 Generation of Multiple Site-Specific Mutations
by Polymerase Chain Reaction
Amom Ruhikanta Meetei and M. R. S. Rao ....................................... 95
13 Phenotypic Expression of Polymerase Chain
Reaction-Generated Random Mutations in a Foreign
Gene After its Introduction into an Acinetobacter
Chromosome by Natural Transformation
David M. Young, Ruben G. Kok,
and L. Nicholas Ornston............................................................... 103
14 Polymerase Chain Reaction-Mediated Mutagenesis
in Sequences Resistant to Homogeneous Amplification
Ross N. Nazar, P. D. Abeyrathne,
and Robert V. A. Intine.................................................................. 117
15 Polymerase Chain Reaction-Based Signature-Tagged
Mutagenesis
Dario E. Lehoux and Roger C. Levesque ....................................... 127
16 High-Throughput Scanning Mutagenesis by Recombination
Polymerase Chain Reaction
Stefan Howorka and Hagan Bayley ................................................. 139
17 In Vitro Scanning-Saturation Mutagenesis
Jennifer A. Maynard, Gang Chen, George Georgiou,
and Brent L. Iverson...................................................................... 149
18 Random Transposon Mutagenesis of Large DNA Molecules
in Escherichia coli
Wolfram Brune ................................................................................... 165
19 Random Chromosomal Gene Disruption Using Cassette
Mutagenesis
French A. Lewis, III and Brian Dougherty ...................................... 173
20. Transplacement Mutagenesis: A Recombination-Based
In Situ Mutagenesis Protocol
Knut Woltjen, M. W. Todd Unger, and Derrick E. Rancourt ......... 189
21 Preparation of Transposon Insertion Lines
and Determination of Insertion Sites
in Arabidopsis Genome
Takuya Ito, Reiko Motohashi, and Kazuo Shinozaki ..................... 209
22 Evolutionary Molecular Engineering by Random Elongation
Mutagenesis
Tomoaki Matsuura, Tetsuya Yomo, and Itaru Urabe .................... 221
23 Random Mutagenesis for Protein Breeding
Chris Fenton, Hao Xu, Evamaria I. Petersen,
Steffen B. Petersen, and M. Raafat El-Gewely ........................... 231
24 DNA Shuffling and Family Shuffling for In Vitro Gene Evolution
Miho Kikuchi and Shigeaki Harayama ............................................ 243
25 Mutagenic Polymerase Chain Reaction of Protein-Coding
Genes for In Vitro Evolution
Ichiro Matsumura and Andrew D. Ellington ................................... 259
Index ............................................................................................................ 269
Invitro Mutagenesis Protocols.rar (1.46 MB, 下载次数: 224)
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绝对零度 +10 +8 感谢分享,生物秀因你而精彩!

总评分: 秀基因 +10 秀蛋白 +8 查看全部评分

共 23 个关于本帖的回复 最后回复于 2017-11-5 10:31

lyacsmb 生物秀才 发表于 2010-6-10 21:10:54 | 显示全部楼层

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抢沙发,真是一本很不错的书呢!多谢楼主。
shane13 生物秀才 发表于 2010-6-10 21:13:28 | 显示全部楼层

生物秀人才网[Job.bbioo.com]

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好东西
shengwuxiufuzho 生物秀举人 发表于 2010-6-10 22:45:29 | 显示全部楼层
thanks
david17857 生物秀举人 发表于 2010-6-10 23:42:09 | 显示全部楼层
Thanks for sharing. It is a good stuff.
uniondavid 生物秀举人 发表于 2010-6-11 14:21:13 | 显示全部楼层
好的,谢谢LZ分享了啊
bolinzhang 生物秀才 发表于 2010-6-11 16:43:16 | 显示全部楼层
谢谢楼主,比较好的书。
rainwater 生物秀举人 发表于 2010-6-11 23:34:13 | 显示全部楼层
thank you
可以 生物秀才 发表于 2010-8-7 09:09:06 | 显示全部楼层
好东西
haoliuzi 生物秀才 发表于 2010-8-10 09:53:47 | 显示全部楼层
谢了
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